FRESH SEMEN MOTILITY OF ETAWA CROSSBREED (PE) WITH DIFFERENT TIME STORAGE AND TEMPERATURE USING VARIOUS DILUENT

Abstract

The purpose of this research is to knowing fresh semen motility of etawa crossbreed (PE) with time storage and temperature using various diluent. The methods is a reseach laboratory using a completely randomized design (CRD) factorial. The diluent treatments are Egg Yolk Tris Aminomethane, Cauda Epididymal Plasma, Ringer, and coconut water. The time storages are 0 hours, 1 hours, 2 hours, 3 hours, 4 hours, 5 hours, 6 hours, and each treatment replicated 10 times. The results showed that the fresh semen motility of crossbreed Etawa (PE) with different time storage and temperature using various diluents is a very significally influence (P <0.01). Egg Yolk Tris Aminomethane is better than other diluent with the average of motility was 55.4 ± 0.52%, and the fresh semen can be stored up to 2 hours at room temperature with the average was 41.6 ± 0.52% and Egg Yolk Tris Aminomethane with 6 hours of time storage can still maintain motility up 40.4 ± 0.52%. In conclution the sperm motility of etawa crossbreed (PE) provides the best results at room temperature with 6 hours time storage.

Key words: fresh semen, motility, etawa crossbreed (PE), time storage, temperature.